mouse rat fgf23 intact elisa kit  (Quidel)

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is down-regulated by pTxA2 in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM of rel. Fgf23 gene expression in UMR-106 ( a , n = 5) and MC3T3-E1 ( b , n = 5) cells treated without or with pTxA2 for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is decreased by TxA2 agonist I-BOP in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM of rel. Fgf23 gene expression in UMR-106 ( a , n = 6) and in MC3T3-E1 ( b , n = 7) cells treated without or with TxA2 agonist I-BOP for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Fgf23 expression is inhibited by TxA2 agonist U46619 in UMR-106 and MC3T3-E1 cells. Arithmetic means ± SEM ( n = 5) of rel. Fgf23 gene expression in UMR-106 ( a ) and MC3T3-E1 ( b ) cells treated without or with TxA2 agonist U46619 for 24 h ( a ) or 6 h ( b ). * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Expressing, Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: C-terminal FGF23 concentration in the supernatant of UMR-106 cells is lowered by TxA2 agonists. Arithmetic means ± SEM of C-terminal FGF23 concentration in the supernatant of UMR-106 cells treated without or with 100 ng/mL pTxA2 ( a , n = 7), 100 ng/mL I-BOP ( b , n = 7) or 5 µ m U46619 ( c , n = 8) for 24 h. * p < 0.05, ** p < 0.01. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Concentration Assay

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: TxA2 antagonist SQ29548 reverses the effect of I-BOP on Fgf23 mRNA transcripts. Arithmetic means ± SEM ( n = 10) of relative Fgf23 gene expression in UMR-106 cells treated without or with 100 ng/mL TxA2 agonist I-BOP in the presence or absence of 1 µ m TxA2 antagonist SQ29548 for 24 h. ** p < 0.01. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Gene Expression

Journal: Kidney & Blood Pressure Research

Article Title: Thromboxane A2 or Activated Platelets Slightly Lower Fgf23 Expression in vitro

doi: 10.1159/000545696

Figure Lengend Snippet: Exposure to activated platelets suppresses Fgf23 mRNA transcripts in UMR-106 cells. Arithmetic means ± SEM ( n = 8) of relative Fgf23 gene expression in UMR-106 cells exposed or not exposed to 100,000/µL freshly isolated human platelets in the presence or absence of 1 U/mL thrombin. * p < 0.05. SEM, standard error of the mean.

Article Snippet: The FGF23 protein concentration was determined using a mouse/rat C-terminal and a mouse/rat intact FGF23 ELISA kit (both from Quidel, San Clemente, CA, USA).

Techniques: Gene Expression, Isolation